FastQCFastQC Report
Fri 10 Feb 2017
SRR3557631.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3557631.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences11733738
Sequences flagged as poor quality0
Sequence length125
%GC46

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT817690.6968708522382211No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT478260.4075938971877504No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT381330.32498595076862974No Hit
GTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGAGGATGGT159630.1360436034961749No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT151650.12924270168636798No Hit
ATCATTAACTGAATCCATAGGTTAATGAGGCGAACCGGGGGAACTGAAAC138110.11770332693639485No Hit
GTTAATGATAGTGTGTCGAAACACACTGGGTTTCCCCATTCGGAAATCGC131620.11217226769508575No Hit
ATACAGGGTGACAGCCCCGTACACAAAAATGCACATGCTGTGAGCTCGAT119060.10146809141298366No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA692650.049.6058271
AACGCAG880350.039.1418956
TATCAAC882450.038.853232
ATCAACG884350.038.5881163
TCAACGC901400.038.0166244
GGTATCA401300.037.8212471
CAACGCA908950.037.6746675
ACGCAGA1007050.034.11697
CGCAGAG1014150.033.8663188
GCAGAGT1075950.031.9542949
AGAGTAC1040150.027.78340510-11
GTACTGG104500.026.7593841
ACGGTAC32600.026.4617793
TACTGGT93000.026.4201772
GGTTCAC89900.025.9414966
AGTACTT609700.024.4774712-13
GAGTACT529250.024.11772212-13
GGACCGT33800.023.9381646
CAGAGTA1063200.023.26688210-11
GTACATG792800.022.9042151
Produced by FastQC (version 0.11.5)