FastQCFastQC Report
Fri 10 Feb 2017
SRR3557626.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3557626.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1426509
Sequences flagged as poor quality0
Sequence length125
%GC44

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT133450.9355005821905085No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT80800.5664177372873217No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT72630.5091450527126012No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA51330.3598294858286909No Hit
CTTATACACATCTCCGAGCCCACGAGACCTCTCTACATCTCGTATGCCGT45370.3180491675832399No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT41840.2933034421794745No Hit
GTACATGGGAAGCAGTGGTATCAAAAAAAAAAAAAAAAAAAAAAAAAAAA40940.286994333719591No Hit
GCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGT38920.2728338902874079No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT37570.2633702275975826No Hit
CTGCACATCTCCGAGCCCACGAGACCTCTCTACATCTCGTATGCCGTCTT32890.23056286360618825RNA PCR Primer, Index 39 (95% over 23bp)
GTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATG29470.20658825145863083No Hit
GCACATCTCCGAGCCCACGAGACCTCTCTACATCTCGTATGCCGTCTTCT28090.1969142851534761RNA PCR Primer, Index 39 (96% over 25bp)
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT27590.19340922489798523No Hit
GTACATGGGCTTTTGTGATGAAGATGGGAGGGGGAAAAAAAAAAAAAAAA27170.1904649742833729No Hit
GTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGAAGCAGTGGTATC25860.18128171641398688No Hit
GAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGG23780.16670066575114492No Hit
GTACATGGGGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAA20240.14188483914226968No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT18100.12688318124876885No Hit
TATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGG18100.12688318124876885No Hit
GAGTACATGGGAAGCAGTGGTATCAAAAAAAAAAAAAAAAAAAAAAAAAA17620.12351832340349762No Hit
TCCATGTACTCTGCGTTGATACCACTGCTTCCATGTACTCTGCGTTGATA17200.12057407278888532No Hit
GTACATGGGCAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAA17020.1193122510969086No Hit
GTACATGGGCCATCTCATCCCTGCACATCTCCGAGCCCACGAGACCTCTC16080.1127227378165858No Hit
GGTTTCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT15530.10886717153554587No Hit
CACAGCATGTGCATTTTTGTGTACGGGGCTGTCACCCTGTATCGCGCGCC15370.10774555225378879No Hit
GCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCC15000.10515180766472555No Hit
GTTAATGATAGTGTGTCGAAACACACTGGGTTTCCCCATTCGGAAATCGC14680.10290856910121142No Hit
GAGTACATGGGCTTTTGTGATGAAGATGGGAGGGGGAAAAAAAAAAAAAA14300.10024472330703837No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCATAG5700.062.628071
GTATAAC5600.061.621551
TAACGCA6500.057.6660044
TATCACG3000.057.5134472
ATAACGC7450.052.7084583
TCACGCA3700.049.8485644
ATAGTAC7850.046.2330784
GTGTTAT8350.044.88971
ATCACGC3800.043.8396533
AGATTAC9450.043.4420132
TAGTACT8600.042.892955
GGTTTCA8200.042.8085821
GCATAGT8450.042.2461552
GAGTTCT10450.042.1316151
GTACAAC3950.040.668611
TACAACG3600.039.6644482
GTATCAC5400.038.5626531
AGTGTAC7850.037.8959663
AGTTCTT11750.037.4702452
CATGGGC72700.036.9910554
Produced by FastQC (version 0.11.5)