FastQCFastQC Report
Fri 10 Feb 2017
SRR3557616.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3557616.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3149855
Sequences flagged as poor quality0
Sequence length125
%GC44

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT405671.2879005541524928No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT207000.6571731079684621No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT152070.4827841281582803No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA130050.4128761482671424No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT105550.3350947900776385No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT83380.264710597789422No Hit
GTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATG65140.20680317030466483No Hit
GTACATGGGAAGCAGTGGTATCAAAAAAAAAAAAAAAAAAAAAAAAAAAA63900.20286648115548175No Hit
GTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGAAGCAGTGGTATC55510.17623033441221897No Hit
GAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGG52900.16794423870305142No Hit
GCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGT52740.16743627881283424No Hit
TCCATGTACTCTGCGTTGATACCACTGCTTCCATGTACTCTGCGTTGATA46970.1491179752718776No Hit
TATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGG44130.14010168722052285No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT41200.13079967173092094No Hit
GTACATGGGCTTTTGTGATGAAGATGGGAGGGGGAAAAAAAAAAAAAAAA41050.13032345933384235No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT40100.1273074474856779No Hit
GCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCC39130.12422794065123632No Hit
ATCATTAACTGAATCCATAGGTTAATGAGGCGAACCGGGGGAACTGAAAC38040.12076746389913186No Hit
GTTAATGATAGTGTGTCGAAACACACTGGGTTTCCCCATTCGGAAATCGC36350.11540213755871302No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCATAG7850.063.3945241
TAACGCA8300.045.1147274
TACAACG6950.041.905132
TCACGCA5850.039.6245774
TAGTACT13650.039.1891375
ATAGTAC14050.036.804324
AGATTAC17200.036.284132
GTACAAC9350.035.910361
TATCACG6150.035.7587662
GCATAGT14300.034.9139632
ATAACGC11300.034.7153363
ACAACGC9100.033.963923
GTATAAC10800.033.864871
ATCTACG5700.033.3680652
GATTACT19300.032.3361173
CATGGGG95450.031.8823174
CATGGGC109050.030.9584034
AGTGTAC18650.030.5948453
ATCACGC7750.029.9101663
AAACGCA12550.029.836835
Produced by FastQC (version 0.11.5)