FastQCFastQC Report
Fri 10 Feb 2017
SRR3557615.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3557615.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1661901
Sequences flagged as poor quality0
Sequence length125
%GC45

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT178321.0729881021793717No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT90950.5472648491095439No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT74180.4463563112363492No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA34890.20994030330326535No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT34730.2089775504076356No Hit
GTACATGGGAAGCAGTGGTATCAAAAAAAAAAAAAAAAAAAAAAAAAAAA27320.16439005692878214No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT26820.16138145412993915No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT21710.13063353352576357No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT18600.11192002411696005No Hit
GTACATGGGCTTTTGTGATGAAGATGGGAGGGGGAAAAAAAAAAAAAAAA17990.10824952870237158No Hit
GTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGAAGCAGTGGTATC17520.10542144207145913No Hit
GTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATG17400.10469937739973681No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCATAG4600.054.754161
TAACGCA4300.045.6155174
TACAACG3200.044.57882
GTATAAC6000.041.9781881
TAGTACT6450.040.5471235
ATAGTAC6350.038.3775524
ATAACGC6200.035.4713023
TATCACG3200.035.291552
TCACGCA3350.033.711334
ACAACGC4150.032.9417653
AAACGCA5650.032.6122175
AACGCAG233250.032.4139946
AGATTAC7950.032.149072
GCATAGT8200.031.1689172
GTACAAC6400.030.9214321
CATGGGG53450.030.6922324
CATGGGT23050.030.6862014
ACATGGG220900.030.1362633
CATGGGC52300.030.0033234
TTCAACG11200.029.7191984