FastQCFastQC Report
Fri 10 Feb 2017
SRR3557613.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3557613.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4001877
Sequences flagged as poor quality0
Sequence length125
%GC45

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT475591.1884173351654737No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT223590.5587128240073346No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT172330.4306229301900083No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA82240.20550356745097365No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT81740.20425415373835826No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT71810.1794407974058173No Hit
ATCATTAACTGAATCCATAGGTTAATGAGGCGAACCGGGGGAACTGAAAC60810.15195369572827952No Hit
GTTAATGATAGTGTGTCGAAACACACTGGGTTTCCCCATTCGGAAATCGC55420.13848501590628598No Hit
GTACATGGGAAGCAGTGGTATCAAAAAAAAAAAAAAAAAAAAAAAAAAAA54990.13741052011343677No Hit
GTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATG45280.11314690581444657No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT42980.10739960273641594No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCATAG10800.048.314371
TAACGCA9200.034.8873334
AACGCAG572850.034.582486
GTATCAA570100.033.7176931
TACAACG7400.032.128472
TAGTACT17450.032.0185
ACGCAGA638550.031.00577
CGCAGAG642250.030.919628
ATAGTAC18350.030.1237244
ACAACGC7900.030.0950243
AAACGCA13500.029.9389765
GCATAGT18000.029.7188382
TATCAAC644050.029.5873262
GCAGAGT672300.029.5464369
ATCAACG646800.029.4431513
TCAACGC662500.029.2209054
CAACGCA668550.028.9742535
TATCACG8200.028.2691362
CATGGGG130650.028.115184
TCACGCA8250.028.0978094