FastQCFastQC Report
Fri 10 Feb 2017
SRR3557610.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3557610.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5034771
Sequences flagged as poor quality0
Sequence length125
%GC45

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT548611.0896424087609944No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT278880.5539080128967137No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT205540.4082410103657148No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA132690.26354723978508654No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT111920.2222941222152904No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT110560.21959290700609818No Hit
GTACATGGGAAGCAGTGGTATCAAAAAAAAAAAAAAAAAAAAAAAAAAAA80030.15895459793504016No Hit
GTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATG62960.12505037468436994No Hit
GTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGAAGCAGTGGTATC59000.11718507157525138No Hit
TCCATGTACTCTGCGTTGATACCACTGCTTCCATGTACTCTGCGTTGATA57340.11388800006991381No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT53630.1065192438742497No Hit
GAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGG52200.1036789955292902No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT50790.10087847093740708No Hit
GCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGT50640.1005805427893344No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCATAG11400.052.080151
TAACGCA11900.042.9547964
TACAACG10350.036.1793172
AGATTAC23850.035.1391222
CATGGGG134700.031.1528134
TATCACG7600.030.50082
ACAACGC11900.030.4679363
TAGTACT20100.030.4579985
AACGCAG708650.030.102456
GATTACT27850.029.8787923
ATAGTAC20400.029.4273664
ATAACGC19150.028.5548063
TCACGCA9650.028.3328784
CATGGGC134650.028.2068544
ACATGGG624600.027.644223
CGCAGAG783050.027.2726848
ACGCAGA782950.027.2230287
GCATAGT22150.027.1024042
GTATCAA762550.026.9203361
CATGGGT58500.026.8230554
Produced by FastQC (version 0.11.5)