FastQCFastQC Report
Fri 10 Feb 2017
SRR3557609.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3557609.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3380350
Sequences flagged as poor quality0
Sequence length125
%GC45

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT353051.044418477376603No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT187220.5538479743221856No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT140860.4167024124720813No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA72440.2142973360746668No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT67820.20063011226648125No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT58600.17335483012114128No Hit
GTACATGGGAAGCAGTGGTATCAAAAAAAAAAAAAAAAAAAAAAAAAAAA53150.15723223926516486No Hit
GTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATG36600.10827281198692443No Hit
GTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGAAGCAGTGGTATC36090.10676409247563122No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT35920.10626118597186682No Hit
ATCATTAACTGAATCCATAGGTTAATGAGGCGAACCGGGGGAACTGAAAC35910.10623160323635127No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCATAG8450.054.644951
TATCACG5150.039.2394332
TACAACG7100.038.5079572
TAACGCA7650.038.0702444
ACAACGC7450.037.4966623
TAGTACT13350.034.2815555
AGATTAC16700.033.09922
ATAGTAC13950.032.807084
GCATAGT14900.031.9120522
GATTACT17650.031.6544063
CATGGGG104550.030.8693034
AACGCAG459750.030.858996
GTACTGG22300.030.3871231
GTATAAC10150.030.1314581
ACATGGG418250.028.961383
TCACGCA7400.028.9149074
CATGGGC94100.028.6127514
GTATCAA476350.028.4888171
AAACGCA11150.028.2521845
TACTGGT20100.028.0917342