FastQCFastQC Report
Fri 10 Feb 2017
SRR3557607.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3557607.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4878488
Sequences flagged as poor quality0
Sequence length125
%GC45

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT499721.0243337689874403No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT259980.5329110166920571No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT186710.3827210397975766No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT91930.18843953290445728No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA80970.16597355574104108No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT75150.15404362991156278No Hit
GTACATGGGAAGCAGTGGTATCAAAAAAAAAAAAAAAAAAAAAAAAAAAA62400.12790848312017986No Hit
GTTAATGATAGTGTGTCGAAACACACTGGGTTTCCCCATTCGGAAATCGC58060.11901228413393658No Hit
ATCATTAACTGAATCCATAGGTTAATGAGGCGAACCGGGGGAACTGAAAC53790.11025957222811657No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT49920.10232678649614388No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCATAG11300.048.823251
AACGCAG582250.034.1971326
TATCACG8550.033.367952
GTATCAA578350.033.11781
GGACCGT11350.031.9439326
ACGCAGA650700.030.5449837
CGCAGAG655950.030.400188
ATAGTAC18750.030.1145744
ACATGGG551900.030.1006473
TAGTACT19550.030.0983625
TAACGCA11600.029.7183284
GTACATG592700.029.5234871
CATGGGG145900.029.2498464
TACATGG595150.029.2314722
CATGGGT81850.029.1918584
GCAGAGT689350.029.0220939
TCACGCA10050.028.9790674
CATGGGC115900.028.974734
ATCAACG661450.028.7725733
TATCAAC666350.028.6234342