FastQCFastQC Report
Fri 10 Feb 2017
SRR3557605.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3557605.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2194359
Sequences flagged as poor quality0
Sequence length125
%GC45

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT227531.03688594254632No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT114060.519787327415432No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT90910.41428954879306445No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT43990.20046856507982513No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA40060.18255900698108193No Hit
GTACATGGGAAGCAGTGGTATCAAAAAAAAAAAAAAAAAAAAAAAAAAAA38260.17435615594348963No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT32750.14924631748952655No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT22770.10376606562554258No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCATAG5450.062.724511
TAGTACT9750.042.6732565
TACAACG4850.040.442182
GCATAGT9950.038.2313162
TAACGCA6650.037.539634
TATCACG3700.036.947792
ATAGTAC10950.035.8254934
GATTACT12100.032.911813
AACGCAG275400.032.7404066
GTATAAC7900.032.643771
CATGGGG62250.032.368514
AGATTAC12250.032.02362
ACAACGC6250.031.3831333
GTATCAA287050.030.1486361
ACATGGG256850.029.5973833
ACGCAGA308850.029.2714317
CGCAGAG309450.029.1954698
ATAACGC9500.028.7803843
GTACATG274750.028.7697851
CATGGGC58150.028.7222824