FastQCFastQC Report
Fri 10 Feb 2017
SRR3557595.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3557595.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6203038
Sequences flagged as poor quality0
Sequence length125
%GC44

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT994671.6035207264569393No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT502950.8108123793534717No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT372750.6009152289571659No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT181650.2928403791819428No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA132920.21428209854590605No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT126640.20415802708285843No Hit
GTACATGGGAAGCAGTGGTATCAAAAAAAAAAAAAAAAAAAAAAAAAAAA126290.20359378743125545No Hit
ATCATTAACTGAATCCATAGGTTAATGAGGCGAACCGGGGGAACTGAAAC105430.16996510419571828No Hit
GTTAATGATAGTGTGTCGAAACACACTGGGTTTCCCCATTCGGAAATCGC99120.15979266933396186No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT96520.1556011747791969No Hit
GTATCAACGCAGAGTACATGGGCTTTTGTGATGAAGATGGGAGGGGGAAA84110.1355948488466458No Hit
GTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAAAAAAAAAAAAAAA81640.1316129290196191No Hit
GTACATGGGCTTTTGTGATGAAGATGGGAGGGGGAAAAAAAAAAAAAAAA72650.11712003053987417No Hit
GTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATG71690.11557240178119174No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT69860.11262223445995333No Hit
GCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT68520.1104620026509591No Hit
GTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGAGGATGGT67180.10830177084196486No Hit
GTACATGGGGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAA64380.10378785362914107No Hit
TCCATGTACTCTGCGTTGATACCACTGCTTCCATGTACTCTGCGTTGATA62820.1012729568962821No Hit
GTGGATGCCCTGGCAGTCAGAGGCGATGAAGGACGTGCTAATCTGCGATA62390.1005797481814556No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCATAG15700.044.693871
GATTACT32150.035.4968153
AACGCAG1068750.035.465776
AGATTAC33450.033.939572
GTATCAA1103500.033.6636051
CATGGGT97050.032.705054
TACAACG12250.032.02412
CGCAGAG1199850.031.6600048
ACGGTAC15400.031.6491643
TCACGCA11100.031.593534
ACGCAGA1199900.031.5546597
GCAGAGT1252100.030.4670079
CATGGGG205200.030.1538314
GACCGTT11850.030.0955317
TAGTACT24400.029.9629365
TATCAAC1242300.029.740872
ATGGGTG58450.029.6939145
TAACGCA15550.029.4326554
ATCAACG1253000.029.4252263
ACAACGC13750.029.3951283
Produced by FastQC (version 0.11.5)