FastQCFastQC Report
Fri 10 Feb 2017
SRR3557589.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3557589.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5237201
Sequences flagged as poor quality0
Sequence length125
%GC44

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT653091.24702107098811No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT354440.6767737193970595No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT245070.4679407950926458No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA168180.3211257310918561No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT145280.2774000845107912No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT130810.24977082223882566No Hit
GTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGAAGCAGTGGTATC100140.19120900649029893No Hit
TCCATGTACTCTGCGTTGATACCACTGCTTCCATGTACTCTGCGTTGATA97510.1861872400925609No Hit
GTACATGGGAAGCAGTGGTATCAAAAAAAAAAAAAAAAAAAAAAAAAAAA87660.16737948381205917No Hit
GTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATG77280.1475597365844847No Hit
ATCATTAACTGAATCCATAGGTTAATGAGGCGAACCGGGGGAACTGAAAC73270.13990297489059517No Hit
GTTAATGATAGTGTGTCGAAACACACTGGGTTTCCCCATTCGGAAATCGC68820.1314060697689472No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT68060.1299549129391826No Hit
GAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGG66340.12667071590339957No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT62590.11951040259863999No Hit
GCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGT60080.11471776622665426No Hit
TATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGG56000.10692734535107588No Hit
GTACATGGGCTTTTGTGATGAAGATGGGAGGGGGAAAAAAAAAAAAAAAA55530.10602991941687936No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCATAG16650.052.5896221
TACAACG11400.044.839082
TAACGCA15100.040.150074
TAGTACT24500.039.059165
ACAACGC13200.038.274383
ATAGTAC25300.035.7096984
TCACGCA9000.035.6627124
GCATAGT25500.035.1965332
AGATTAC30650.034.3246352
GTATAAC16650.034.2192731
CATGGGT88150.032.8374754
GATTACT31950.032.369913
TATCACG9950.032.257732
ATCACGC10700.031.6631553
GTACAAC18350.031.375931
CATGGGG152800.030.535814
AAACGCA21150.029.5081545
CATGGGC152000.028.858644
AGTGTAC27200.028.8448433
GGTTTCA31600.028.4685671