FastQCFastQC Report
Fri 10 Feb 2017
SRR3557585.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3557585.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2980610
Sequences flagged as poor quality0
Sequence length125
%GC45

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT421701.4148110621651273No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT214390.7192822945638644No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT166240.557738181110578No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT80260.26927373926813636No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA69950.2346835043833309No Hit
GTACATGGGAAGCAGTGGTATCAAAAAAAAAAAAAAAAAAAAAAAAAAAA60440.20277728384458216No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT59470.1995229164499884No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT41520.13930034456034165No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT34780.11668752369481414No Hit
GTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATG34420.11547971723908865No Hit
GTACATGGGCTTTTGTGATGAAGATGGGAGGGGGAAAAAAAAAAAAAAAA34380.11534551652178582No Hit
GTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAAAAAAAAAAAAAAA31240.10481076021351333No Hit
GCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGT30630.10276419927464511No Hit
ATCATTAACTGAATCCATAGGTTAATGAGGCGAACCGGGGGAACTGAAAC29810.10001308456993703No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCATAG7450.054.7403561
TAGTACT11450.037.8934755
ATAGTAC11450.037.3743864
TATCACG3850.033.9632342
AACGCAG483350.033.7418946
AGATTAC16150.033.1220362
GCATAGT13050.032.7920882
GATTACT16700.032.3870963
CATGGGG98850.031.5066134
GGACCGT7850.031.0428286
GTATCAA508500.030.9240761
AAACGCA10700.030.551045
ACGCAGA536800.030.3268037
CGCAGAG540350.030.1715588
ACGCATA3400.029.9864441
TACAACG5550.029.9855582
TAACGCA8000.029.717834
TACGCAG5800.029.717835
GTATAAC9400.029.3484361
GCAGAGT562350.029.022919