FastQCFastQC Report
Fri 10 Feb 2017
SRR3557583.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3557583.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4117890
Sequences flagged as poor quality0
Sequence length125
%GC43

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[WARN]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT693341.6837263744296231No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT370820.9005097270689602No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT274320.6661664104674967No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA169990.4128085014412721No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT144610.3511749949610116No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT130510.3169341580275335No Hit
ATCATTAACTGAATCCATAGGTTAATGAGGCGAACCGGGGGAACTGAAAC85600.2078734497521789No Hit
GTTAATGATAGTGTGTCGAAACACACTGGGTTTCCCCATTCGGAAATCGC80790.1961927103443754No Hit
GTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGAAGCAGTGGTATC80570.19565845615108707No Hit
GTACATGGGAAGCAGTGGTATCAAAAAAAAAAAAAAAAAAAAAAAAAAAA79750.19366714506701246No Hit
TCCATGTACTCTGCGTTGATACCACTGCTTCCATGTACTCTGCGTTGATA79290.19255006811740966No Hit
GTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATG74480.18086932870960615No Hit
GAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGG69650.1691400207387764No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT68930.16739155246983287No Hit
GCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGT62280.15124250526361802No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT60950.14801269582237506No Hit
GTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGAGGATGGT56510.13723047483055642No Hit
TATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGG53800.13064943454050496No Hit
GTGGATGCCCTGGCAGTCAGAGGCGATGAAGGACGTGCTAATCTGCGATA51360.12472406985130735No Hit
GTACATGGGCTTTTGTGATGAAGATGGGAGGGGGAAAAAAAAAAAAAAAA50880.12355842433867831No Hit
GTATCAACGCAGAGTACATGGGCTTTTGTGATGAAGATGGGAGGGGGAAA50260.1220527988848658No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT47300.1148646515569867No Hit
GCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT46390.11265478193929417No Hit
GCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCC46330.11250907625021551No Hit
GTACATGGGGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAA45020.10932783537199876No Hit
ATACAGGGTGACAGCCCCGTACACAAAAATGCACATGCTGTGAGCTCGAT43510.1056609088635199No Hit
GTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAAAAAAAAAAAAAAA43450.10551520317444128No Hit
GGATACCACGTGTCCCGCCCTACTCATCGAGCTCACAGCATGTGCATTTT42270.10264965795589488No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCATAG8350.053.511911
GACCGTT6700.037.7271277
TATCACG7750.037.166742
TCACGCA7700.036.644654
TACAACG8150.036.0638772
AGATTAC17600.034.0680732
ACAACGC8600.033.493283
GTATAAC11350.033.1518251
GGACCGT11800.032.3810846
ATCTACG5850.032.155422
CATGGGT66500.031.9997774
GCATAGT14700.031.991362
AGTGTAC18650.031.8348333
TAACGCA11200.031.4914954
ATAACGC14000.030.2318363
AAACGCA13300.030.0550385
ATAGTAC15550.029.8646034
GTGTACT20950.029.4622084
CATGGGG134050.029.1179834
ACGGTAC9700.029.0890523
Produced by FastQC (version 0.11.5)