FastQCFastQC Report
Fri 10 Feb 2017
SRR3557580.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3557580.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5698884
Sequences flagged as poor quality0
Sequence length125
%GC45

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[WARN]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT732651.2856025846463974No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT382770.6716578193204142No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT285210.5004664071070757No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA165570.29053056703733576No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT146500.25706787504360507No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT140160.2459428898710695No Hit
GTACATGGGAAGCAGTGGTATCAAAAAAAAAAAAAAAAAAAAAAAAAAAA81550.14309819255840264No Hit
GTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATG76470.13418416658419438No Hit
GTTAATGATAGTGTGTCGAAACACACTGGGTTTCCCCATTCGGAAATCGC75170.13190301820496783No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT74990.1315871668909211No Hit
GTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGAAGCAGTGGTATC70980.1245507015057685No Hit
TCCATGTACTCTGCGTTGATACCACTGCTTCCATGTACTCTGCGTTGATA70540.12377862051587643No Hit
ATCATTAACTGAATCCATAGGTTAATGAGGCGAACCGGGGGAACTGAAAC68650.12046218171838556No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT64630.11340816903800813No Hit
GAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGG61910.10863530473685726No Hit
GTATCAACGCAGAGTACATGGGCTTTTGTGATGAAGATGGGAGGGGGAAA60180.10559962266296348No Hit
GTACATGGGCTTTTGTGATGAAGATGGGAGGGGGAAAAAAAAAAAAAAAA57720.1012829880376579No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCATAG9900.053.4459041
AACGCAG893350.031.8015986
TATCACG9550.031.3921172
CATGGGG190550.031.2811584
TAGTACT17300.031.2604715
CATGGGT75850.031.1547494
GTATCAA911450.030.4579221
ATAGTAC18050.029.961564
TAACGCA11050.029.790624
ACGCAGA991950.028.5931057
CGCAGAG992750.028.5463818
TCACGCA11150.028.4690324
TACAACG9300.027.8114432
ACATGGG787500.027.5889513
GCAGAGT1043350.027.1957539
CATGGGC165150.027.1937184
AGATTAC23000.027.0914232
TATCAAC1031150.026.8562322
ATCACGC11700.026.6283233
GGACCGT13500.026.5613336