FastQCFastQC Report
Fri 10 Feb 2017
SRR3557570.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3557570.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences11650850
Sequences flagged as poor quality0
Sequence length125
%GC46

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[WARN]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT1036630.8897462416905205No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT530690.45549466347948864No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT395890.3397949505830047No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT179540.15410034461004993No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA169900.1458262701862954No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT163620.1404361055201981No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA1077500.036.7178151
AACGCAG1154850.034.7184646
ACGCAGA1285700.031.2124827
CGCAGAG1296050.031.067548
TATCAAC1271000.030.9583782
ATCAACG1268550.030.8513573
TCAACGC1291650.030.640924
CAACGCA1306050.030.3165875
GCAGAGT1374450.029.3339069
CATGGGT152000.029.119874
ACATGGG1073800.028.4654453
TAACGCA18200.028.4216524
GTACATG1159800.027.8068451
TACATGG1165400.027.5798282
CATGGGG342750.027.1653774
CGCATAG17200.027.0008661
AGTACTT689750.024.97399512-13
AGAGTAC1343150.024.53390110-11
TATCACG15250.023.8979072
GAGTACT617500.023.8218312-13
Produced by FastQC (version 0.11.5)