FastQCFastQC Report
Fri 10 Feb 2017
SRR3557560.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3557560.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4732894
Sequences flagged as poor quality0
Sequence length125
%GC46

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[WARN]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT450780.952440515253458No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT231740.4896369958845476No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT168750.3565471781113205No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA154360.3261429476341537No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT140720.29732337128192604No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT88650.18730611756781368No Hit
GTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATG69000.1457881794944066No Hit
GTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGAAGCAGTGGTATC60380.1275752214184387No Hit
GAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGG59410.12552573541685066No Hit
TCCATGTACTCTGCGTTGATACCACTGCTTCCATGTACTCTGCGTTGATA59190.12506090354020183No Hit
GTTAATGATAGTGTGTCGAAACACACTGGGTTTCCCCATTCGGAAATCGC56460.11929276252542313No Hit
GCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGT52950.11187658122070766No Hit
ATCATTAACTGAATCCATAGGTTAATGAGGCGAACCGGGGGAACTGAAAC50860.10746067839254377No Hit
TATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGG48440.1023475277494066No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCATAG10000.040.563291
TAACGCA9050.037.6727034
CATGGGG137950.030.1688634
GTATAAC13200.029.8390481
TACAACG8350.029.56722
AACGCAG637650.028.6329776
CATGGGC129200.028.1172834
GTATCAA647650.027.2583181
ACATGGG588300.027.0481033
TAGTACT15950.026.9035475
ATAACGC15300.026.8939133
ATAGTAC15300.026.8939134
ACAACGC8750.026.871963
CATGGGT51250.026.4950964
ACGCAGA704750.025.8901167
CGCAGAG709450.025.7600278
TACATGG662450.025.6709962
GTACATG666300.025.613051
AGATTAC18150.025.585732
AAACGCA14000.025.192465
Produced by FastQC (version 0.11.5)