FastQCFastQC Report
Fri 10 Feb 2017
SRR3557552.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3557552.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7878643
Sequences flagged as poor quality0
Sequence length125
%GC45

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[WARN]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT870561.1049618570101476No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT416750.528961649867877No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT337710.4286398051034931No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT192220.2439760248052869No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA188810.23964786829407045No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT151430.19220314970484131No Hit
ATCATTAACTGAATCCATAGGTTAATGAGGCGAACCGGGGGAACTGAAAC101170.1284104381934808No Hit
GTTAATGATAGTGTGTCGAAACACACTGGGTTTCCCCATTCGGAAATCGC93250.11835794565130062No Hit
GTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATG92810.11779947384340173No Hit
TCCATGTACTCTGCGTTGATACCACTGCTTCCATGTACTCTGCGTTGATA87550.11112319723079216No Hit
GTACATGGGAAGCAGTGGTATCAAAAAAAAAAAAAAAAAAAAAAAAAAAA86780.11014587156696909No Hit
GAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGG81640.10362190544742286No Hit
GTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGAAGCAGTGGTATC79520.10093108673663725No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AACGCAG1083750.031.5601636
GTATCAA1088750.030.938441
CGCATAG13100.030.5040841
CGCAGAG1206350.028.3722258
ACGCAGA1208550.028.2914057
TACAACG12400.027.9576762
TATCAAC1230650.027.2724672
TATCACG12100.027.1940212
ATCAACG1233750.027.0515373
GCAGAGT1271250.026.9006529
TCAACGC1255100.026.900364
CAACGCA1267100.026.7244345
CATGGGT90250.026.3029794
CATGGGG239850.025.7474234
GTACTGG53150.024.8770921
ACATGGG971050.024.5853273
AGTACTT586950.024.2762412-13
GTACATG1065350.023.7134911
TACATGG1061550.023.6904932
GAGTACT538150.023.14199612-13
Produced by FastQC (version 0.11.5)