FastQCFastQC Report
Fri 10 Feb 2017
SRR3557548.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3557548.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6677631
Sequences flagged as poor quality0
Sequence length125
%GC46

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[WARN]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT710211.0635658064963458No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT373330.5590755164518674No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT261450.39153106842830937No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA190350.28505618234969854No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT171540.25688750995674964No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT143000.21414780181774046No Hit
GTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATG83030.12434050339109784No Hit
GTTAATGATAGTGTGTCGAAACACACTGGGTTTCCCCATTCGGAAATCGC74610.11173124121413716No Hit
GTACATGGGAAGCAGTGGTATCAAAAAAAAAAAAAAAAAAAAAAAAAAAA73800.11051823618286186No Hit
ATCATTAACTGAATCCATAGGTTAATGAGGCGAACCGGGGGAACTGAAAC71280.10674444275222755No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT71010.10634010774180246No Hit
GAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGG70980.10629518162953298No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCATAG13550.046.420631
TAACGCA13050.034.681774
CATGGGG200050.031.703264
AACGCAG890050.030.8670676
CATGGGT83750.030.5299074
TACAACG11950.030.4961382
GTATCAA890250.030.1766321
CATGGGC180400.028.8681014
ACATGGG827750.028.7663063
TAGTACT24000.028.6546865
ACAACGC13950.028.6520523
GTACATG911050.027.790681
ATAGTAC22850.027.7816854
ACGCAGA987850.027.7575867
CGCAGAG991900.027.667958
TACATGG910700.027.6435472
TATCAAC1005250.026.6280862
ATCAACG1004750.026.3839363
GCAGAGT1040550.026.3630649
TCAACGC1023200.026.258614
Produced by FastQC (version 0.11.5)