FastQCFastQC Report
Fri 10 Feb 2017
SRR3557547.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3557547.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5612494
Sequences flagged as poor quality0
Sequence length125
%GC45

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[WARN]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT632411.1267896232940293No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT341620.6086777108358601No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT245330.43711405303952217No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT116660.20785768323315804No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA109810.19565277040830686No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT95490.17013826651752323No Hit
GTACATGGGAAGCAGTGGTATCAAAAAAAAAAAAAAAAAAAAAAAAAAAA66370.11825402396866704No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT63010.11226738059764518No Hit
ATCATTAACTGAATCCATAGGTTAATGAGGCGAACCGGGGGAACTGAAAC62620.111572502349223No Hit
GTTAATGATAGTGTGTCGAAACACACTGGGTTTCCCCATTCGGAAATCGC58960.1050513372486456No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT56880.1013453199237273No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCATAG10000.047.0320631
TAACGCA11400.032.4852034
AACGCAG716400.032.435546
TACAACG9850.031.6292932
GTATCAA717600.031.353071
TATCACG9050.029.8785212
ACGCAGA797550.029.127887
CATGGGG167200.029.0749574
CGCAGAG801300.028.9915668
CATGGGT80400.028.587134
ACATGGG632900.028.2721883
TAGTACT18350.028.190095
ACGGTAC11650.027.751513
GCAGAGT840900.027.61239
TATCAAC819550.027.4135382
TCAACGC829450.027.4052544
ATCAACG816200.027.3388023
CAACGCA839050.027.203795
GTACATG702850.027.142891
TACATGG700600.027.134372