FastQCFastQC Report
Fri 10 Feb 2017
SRR3557546.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3557546.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6178306
Sequences flagged as poor quality0
Sequence length125
%GC44

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[WARN]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT876841.4192239749860236No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT454690.7359460667697586No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT338020.5471078965658225No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA193450.3131117170305258No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT171550.277665107555372No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT161180.2608805714705617No Hit
GTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATG91650.1483416328035549No Hit
GTTAATGATAGTGTGTCGAAACACACTGGGTTTCCCCATTCGGAAATCGC88180.1427252065533821No Hit
ATCATTAACTGAATCCATAGGTTAATGAGGCGAACCGGGGGAACTGAAAC86310.13969848693153106No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT83860.13573299865691338No Hit
TCCATGTACTCTGCGTTGATACCACTGCTTCCATGTACTCTGCGTTGATA83340.13489134400270883No Hit
GTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGAAGCAGTGGTATC82160.13298143536432155No Hit
GTACATGGGAAGCAGTGGTATCAAAAAAAAAAAAAAAAAAAAAAAAAAAA81590.13205885237798193No Hit
GAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGG73960.11970918889417262No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT72560.11744319559439109No Hit
GTATCAACGCAGAGTACATGGGCTTTTGTGATGAAGATGGGAGGGGGAAA70370.1138985346468757No Hit
TATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGG65530.10606467209620242No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCATAG11000.053.980151
TACAACG11400.034.5490952
CATGGGT107500.033.9039234
TATCACG9250.033.682232
ACAACGC12550.033.2568663
AACGCAG1055850.032.464416
GTATCAA1044750.032.3451961
CATGGGG216700.030.6539364
TAACGCA12950.029.9599364
CGCAGAG1164950.029.439198
ACGCAGA1165550.029.4189917
ATAGTAC20450.029.3206484
TAGTACT20750.029.1800375
TCACGCA11200.028.8676474
GCATAGT21350.028.635332
GCAGAGT1211350.028.3358029
TATCAAC1189850.028.2648332
ATGGGTG65800.028.0524275
ATCAACG1194700.027.8647023
TCAACGC1218550.027.7100814
Produced by FastQC (version 0.11.5)