FastQCFastQC Report
Fri 10 Feb 2017
SRR3557543.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3557543.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2568382
Sequences flagged as poor quality0
Sequence length125
%GC46

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[WARN]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT278111.0828217920854453No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT136750.532436374339954No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT104390.4064426553370955No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA54750.21316922482714798No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT51700.20129404426600092No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT43970.17119727517168395No Hit
GTACATGGGAAGCAGTGGTATCAAAAAAAAAAAAAAAAAAAAAAAAAAAA31580.12295678758066361No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT29070.1131840980041131No Hit
GTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATG26130.101737202643532No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCATAG4800.048.9896131
TACAACG4800.036.7393462
TAACGCA4650.036.6603364
AACGCAG327900.033.505756
ACAACGC5350.032.9624063
CATGGGG83350.032.794334
CATGGGT35650.032.3182564
GTATCAA333300.032.224671
ACATGGG316400.031.5094473
GTACATG345450.030.4616261
TACATGG346300.030.3165932
CATGGGC78050.030.2764234
TAGTACT8350.030.2714615
ACGCAGA367800.029.8709457
CGCAGAG368300.029.7984738
ATAGTAC8800.028.7234884
GCAGAGT385750.028.5266869
TATCAAC378150.028.3072222
ATCAACG376900.028.1359653
TCAACGC382750.028.0745244