FastQCFastQC Report
Fri 10 Feb 2017
SRR3557540.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3557540.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8782513
Sequences flagged as poor quality0
Sequence length125
%GC45

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT1170701.3329897718340982No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT579470.6597997634617792No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT444350.5059485821427192No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA222160.2529572116773411No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT217490.24763982700623388No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT206060.23462532876410203No Hit
ATCATTAACTGAATCCATAGGTTAATGAGGCGAACCGGGGGAACTGAAAC199800.22749752832702894No Hit
GTTAATGATAGTGTGTCGAAACACACTGGGTTTCCCCATTCGGAAATCGC165540.18848819238867054No Hit
GTACATGGGAAGCAGTGGTATCAAAAAAAAAAAAAAAAAAAAAAAAAAAA131460.14968380917853466No Hit
GTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGAGGATGGT128770.14662090451787546No Hit
GTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATG101720.11582106397109801No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT101570.11565027003091256No Hit
GAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGG98720.11240518516738889No Hit
GTATCAACGCAGAGTACATGGGCTTTTGTGATGAAGATGGGAGGGGGAAA94770.10790761140917185No Hit
ATACAGGGTGACAGCCCCGTACACAAAAATGCACATGCTGTGAGCTCGAT94050.10708780049628165No Hit
GTGGATGCCCTGGCAGTCAGAGGCGATGAAGGACGTGCTAATCTGCGATA93870.1068828477680591No Hit
GCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGT92910.10578976655087216No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GACCGTT18400.037.8318827
GTATCAA1319600.034.470871
AACGCAG1358650.033.7144936
CGCATAG18150.032.782381
TAACGCA15800.030.8783514
ACGGTAC24850.030.4070743
CGCAGAG1516550.030.2669838
ACGCAGA1515450.030.2261357
TATCAAC1501600.030.196392
TTAGGAC28800.030.1618333
TACAACG13100.029.9757462
ATCAACG1507350.029.793063
TCAACGC1534050.029.5460034
GGACCGT31300.029.463026
CAACGCA1547700.029.3541185
GCAGAGT1584200.029.0007829
CATGGGG281200.027.6539764
CATGGGC270900.027.4974674
GTACTGG74100.026.819171
ACATGGG1205050.026.4048483
Produced by FastQC (version 0.11.5)