FastQCFastQC Report
Fri 10 Feb 2017
SRR3557538.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3557538.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1506378
Sequences flagged as poor quality0
Sequence length125
%GC45

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT135320.8983137034661951No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT67820.45021900213625No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA61400.4076002172097575No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT58720.3898091979569537No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT46320.30749254171263785No Hit
GCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGT37130.24648527793156833No Hit
GTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATG28710.19058961296566998No Hit
GTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGAAGCAGTGGTATC28620.18999215336389672No Hit
GAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGG27620.1833537133441938No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT27390.18182687213966214No Hit
TCCATGTACTCTGCGTTGATACCACTGCTTCCATGTACTCTGCGTTGATA21960.14578014283267546No Hit
TATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGG20200.1340964883979984No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT19460.12918404278341825No Hit
GCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCC18350.12181537436154802No Hit
GTACATGGGAAGCAGTGGTATCAAAAAAAAAAAAAAAAAAAAAAAAAAAA16220.10767549711958088No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAACGCA3750.044.4253544
CGCATAG3150.041.5557021
TACAACG2650.040.4139022
TATCACG2400.037.18642
TCACGCA2600.036.6143044
ATCACGC2550.034.9989663
GTATAAC5650.033.6992341
GGACCGT3450.032.766066
CATGGGG43600.029.8855824
ACAACGC3750.028.5591563
ATAACGC7150.028.292873
ACGGTAC3350.026.6410063
CATGGGC40900.026.6214644
GCATAGT5450.026.201062
GATTACT6600.026.1431663
GTGTACT8450.026.0524854
AGATTAC6650.025.94662
CACGGTA3500.025.4992452
GTACTAA2151.2325108E-724.9070661
CATGGGT22000.024.8810834
Produced by FastQC (version 0.11.5)