FastQCFastQC Report
Fri 10 Feb 2017
SRR3557527.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3557527.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7596585
Sequences flagged as poor quality0
Sequence length125
%GC47

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT555920.7318025138927557No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT288360.3795916191288585No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT215160.2832325314598599No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA131370.17293296922235452No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT119730.15761029462580883No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT108110.14231394764884484No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAACGCA13600.034.9980284
TATCACG10850.033.4497262
AACGCAG743300.030.2722136
GTATCAA739900.029.8500161
CGCATAG13050.028.7236541
ACGCAGA823900.027.3035377
CGCAGAG832850.027.074428
ATCAACG835000.026.1785263
TCAACGC845850.026.1733194
CAACGCA853600.026.0329235
CATGGGG199250.025.94864
TATCAAC849250.025.9144062
GCAGAGT882500.025.5714249
ACATGGG685300.025.5593343
CATGGGT87150.025.1230814
GTACATG769200.024.5359861
TACATGG771800.024.236522
AGTACTT405650.023.56221212-13
TCACGCA15800.022.9702244
GAGTACT364350.022.2078712-13
Produced by FastQC (version 0.11.5)