FastQCFastQC Report
Fri 10 Feb 2017
SRR3557516.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3557516.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences12156852
Sequences flagged as poor quality0
Sequence length125
%GC46

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT1116510.9184203278940962No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT574500.47257299833871463No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT417650.34355111010646505No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT208910.171845474469871No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT202400.16649046973673776No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA197830.16273127286570568No Hit
ATCATTAACTGAATCCATAGGTTAATGAGGCGAACCGGGGGAACTGAAAC143850.11832833039342751No Hit
GTTAATGATAGTGTGTCGAAACACACTGGGTTTCCCCATTCGGAAATCGC124630.10251831641941515No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA1275000.034.6582261
AACGCAG1301050.034.1046526
ACGCAGA1452000.030.5181547
CGCAGAG1461450.030.406318
TATCAAC1476650.029.7874772
ATCAACG1474900.029.4395963
TCAACGC1497800.029.3072724
CAACGCA1507100.029.1813325
GCAGAGT1556150.028.5979799
CATGGGT159500.027.1930184
CATGGGG328150.027.1237534
ACATGGG1186150.026.1279493
GTACATG1302350.025.4100971
TACATGG1303900.025.1418882
CGCATAG20250.024.974511
AGTACTT776050.024.44837612-13
TAACGCA18450.024.1855224
AGAGTAC1522800.024.04752710-11
GAGTACT688550.023.0318312-13
TATCACG16800.023.0194362
Produced by FastQC (version 0.11.5)