FastQCFastQC Report
Fri 10 Feb 2017
SRR3557514.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3557514.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4403574
Sequences flagged as poor quality0
Sequence length125
%GC44

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT729731.6571312302234502No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT366460.8321876730128754No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT292170.6634837974790477No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT125060.28399659004254274No Hit
ATCATTAACTGAATCCATAGGTTAATGAGGCGAACCGGGGGAACTGAAAC77120.1751304735653358No Hit
GTTAATGATAGTGTGTCGAAACACACTGGGTTTCCCCATTCGGAAATCGC73530.16697800468437682No Hit
GTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGAGGATGGT69840.15859844753375327No Hit
GTATCAACGCAGAGTACATGGGCTTTTGTGATGAAGATGGGAGGGGGAAA66060.15001451094043158No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT64590.14667631337636203No Hit
GTACATGGGAAGCAGTGGTATCAAAAAAAAAAAAAAAAAAAAAAAAAAAA63360.1438831276594875No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA60290.13691151778078445No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT58150.13205182880996208No Hit
ATACAGGGTGACAGCCCCGTACACAAAAATGCACATGCTGTGAGCTCGAT51480.11690504122333359No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTC48670.11052386084575846No Hit
GCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT47900.10877528116934108No Hit
GTACATGGGCTTTTGTGATGAAGATGGGAGGGGGAAAAAAAAAAAAAAAA47360.10754900451315227No Hit
GTGGATGCCCTGGCAGTCAGAGGCGATGAAGGACGTGCTAATCTGCGATA46450.10548250125920446No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA549800.054.5326961
TATCAAC687400.043.50982
AACGCAG695550.042.6834956
ATCAACG702350.042.100823
TCAACGC719950.041.1955764
CAACGCA733250.040.4726945
GACCGTT8650.039.8932727
CGCAGAG795750.037.3013578
ACGCAGA795950.037.254617
GGTATCA295000.036.7690161
GCAGAGT832900.035.6518869
CATGGGT96950.032.770364
GTACTGG33950.031.8969331
GGACCGT14550.031.8947626
GTACATG655350.030.4877051
TACATGG652650.030.3564952
ACATGGG643500.030.187173
AGAGTAC831650.029.33485610-11
CATGGGG180850.029.2134084
TACTGGT33450.029.1699352
Produced by FastQC (version 0.11.5)