FastQCFastQC Report
Fri 10 Feb 2017
SRR3557510.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3557510.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8168922
Sequences flagged as poor quality0
Sequence length125
%GC45

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT829531.0154705847356604No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT390440.4779578015312179No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT352590.4316236585439303No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA329130.4029050589539232No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT313640.38394294865344536No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT150130.18378189925182295No Hit
TCCATGTACTCTGCGTTGATACCACTGCTTCCATGTACTCTGCGTTGATA147250.1802563422689065No Hit
GAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGG145280.17784476336045318No Hit
GTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATG137790.16867586690141No Hit
GTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGAAGCAGTGGTATC136320.16687636385804638No Hit
ATCATTAACTGAATCCATAGGTTAATGAGGCGAACCGGGGGAACTGAAAC129630.15868678878314665No Hit
GTTAATGATAGTGTGTCGAAACACACTGGGTTTCCCCATTCGGAAATCGC127260.15578554918262164No Hit
GCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCC120370.14735114376168607No Hit
GCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGT113760.1392595008252007No Hit
CATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATAC97940.11989342045376367No Hit
TATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGG94550.11574354608845573No Hit
GTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGAGGATGGT90100.11029607088915772No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CATGGGT120500.029.9211884
ACGGTAC17500.027.1985173
CATGGGG222050.027.1158394
TACTGGT53200.027.0643122
GTACTGG64100.026.2683221
AACGCAG1333600.026.1745056
TAACGCA15700.026.1482564
GTATCAA1358000.025.4291321
GACCGTT14600.025.2654367
ATAACGC19600.024.89153
ATGGGTG75400.024.5401385
ACAACGC16250.024.1648373
CGCAGAG1461600.023.8985488
ACGCAGA1458750.023.8677467
AGTACTT611750.023.72561512-13
GGACCGT21950.023.581576
GGTTCAC57350.023.549436
CGCATAG16000.023.4274221
GAGTACT544300.023.13505612-13
TATCAAC1492500.023.0891272
Produced by FastQC (version 0.11.5)