FastQCFastQC Report
Fri 10 Feb 2017
SRR3557509.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3557509.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3308530
Sequences flagged as poor quality0
Sequence length125
%GC46

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT294160.8890957615617812No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT138820.41958211048411226No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT121680.3677766258731219No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA102830.3108026827624353No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT99580.3009795891226617No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT53530.1617939084729472No Hit
TCCATGTACTCTGCGTTGATACCACTGCTTCCATGTACTCTGCGTTGATA53110.1605244625256534No Hit
GTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGAAGCAGTGGTATC50890.15381453394710037No Hit
GTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATG46490.14051557640402235No Hit
GAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGG45720.13818825883398367No Hit
GCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGT44400.13419857157106024No Hit
GCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCC34750.10503153968680955No Hit
GTACATGGGAAGCAGTGGTATCAAAAAAAAAAAAAAAAAAAAAAAAAAAA34610.1046083910377116No Hit
ATCATTAACTGAATCCATAGGTTAATGAGGCGAACCGGGGGAACTGAAAC33920.10252287269572893No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCATAG6700.049.727651
TAACGCA7700.038.6334954
ATAGTAC11900.028.4978834
TAGTACT11900.028.4978835
GTACTGG20550.028.3725871
TACTGGT16450.028.210672
TACAACG5950.027.9979212
CATGGGG86800.027.8285774
GTATAAC12300.026.6037161
AACGCAG488600.026.2043576
TATCACG4450.025.4026072
ACAACGC6400.025.0996973
ATAACGC14100.024.8953133
AAACGCA10300.024.837965
GCATAGT13050.024.6188622
CATGGGT42150.024.5604534
ACATGGG418650.024.5286943
AGATTAC14600.024.450242
GTATCAA510100.024.201791
ACGCAGA534950.023.900557
Produced by FastQC (version 0.11.5)