FastQCFastQC Report
Fri 10 Feb 2017
SRR3557501.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3557501.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4299239
Sequences flagged as poor quality0
Sequence length125
%GC46

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT492961.1466215299963551No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT249130.5794746465595423No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT194610.45266150590837123No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA116950.2720248862647552No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT111120.2584643468297529No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT91370.2125259842497707No Hit
GTACATGGGAAGCAGTGGTATCAAAAAAAAAAAAAAAAAAAAAAAAAAAA53780.12509190580007298No Hit
GTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATG47270.10994969109649405No Hit
GAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGG46860.10899603394926405No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT46690.10860061513211988No Hit
GCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGT43750.10176219558856811No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCATAG9000.040.3277441
TATCACG5800.033.8518452
TACAACG6800.032.3734662
AACGCAG599900.031.7665026
TAACGCA9600.030.9881154
GTATCAA617600.030.3761861
CATGGGT57350.029.9820144
CATGGGG122000.029.4559824
ACGCAGA668450.028.5088257
CGCAGAG670600.028.4795288
CATGGGC117250.028.0613564
GCAGAGT701350.027.3072229
TAGTACT13800.027.161445
TATCAAC696950.026.7116262
ACATGGG548850.026.7105873
TCAACGC704350.026.6844024
ATCAACG694150.026.5622353
CAACGCA710300.026.5443295
GTACATG596400.026.1384751
TACATGG593650.026.097982