FastQCFastQC Report
Fri 10 Feb 2017
SRR3557494.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3557494.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences9982539
Sequences flagged as poor quality0
Sequence length125
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT1493251.4958619245063804No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT730420.7316976172094094No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT583970.5849914535770909No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT253720.2541637954031535No Hit
GTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGAGGATGGT186960.18728702186888527No Hit
GTTAATGATAGTGTGTCGAAACACACTGGGTTTCCCCATTCGGAAATCGC182830.18314979786204694No Hit
ATCATTAACTGAATCCATAGGTTAATGAGGCGAACCGGGGGAACTGAAAC181220.1815369817237879No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT140880.1411264208434347No Hit
GTGGATGCCCTGGCAGTCAGAGGCGATGAAGGACGTGCTAATCTGCGATA129990.13021737255421692No Hit
ATACAGGGTGACAGCCCCGTACACAAAAATGCACATGCTGTGAGCTCGAT129910.13013723262188107No Hit
GTACATGGGAAGCAGTGGTATCAAAAAAAAAAAAAAAAAAAAAAAAAAAA127610.12783320956722535No Hit
GTATCAACGCAGAGTACATGGGCTTTTGTGATGAAGATGGGAGGGGGAAA124660.1248780495623408No Hit
CCTATATAGTATAGCTTCCCATCTTCTTTGAGAGTTGTTGGTTTATGCTC111540.11173510065926114No Hit
GGATACCACGTGTCCCGCCCTACTCATCGAGCTCACAGCATGTGCATTTT107240.10742757929620911No Hit
ATATAGTATAGCTTCCCATCTTCTTTGAGAGTTGTTGGTTTATGCTCATC106180.10636572519275908No Hit
ATTTAGGGCTATTGATATTTAACAAATATCCAGCAAAGGTTTTTCCAGGA102360.10253904342372216No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA1048100.053.4545941
AACGCAG1267150.044.3021856
TATCAAC1291800.043.347362
ATCAACG1328950.042.0818823
TCAACGC1369700.040.9602244
CAACGCA1375550.040.7374235
ACGCAGA1460750.038.329597
CGCAGAG1461350.038.301838
GGTATCA572400.037.273751
GCAGAGT1535300.036.487979
TACTGGT77700.036.2211532
GACCGTT20100.034.3349347
ACGGTAC29400.034.202793
GTACTGG87750.034.0463181
GGTTCAC82750.032.356076
ACTGGTT88950.030.904253
TACATGG1130600.029.7871932
GTACATG1132400.029.7513621
AGAGTAC1511950.029.73852210-11
ACATGGG1119250.029.648023
Produced by FastQC (version 0.11.5)