FastQCFastQC Report
Fri 10 Feb 2017
SRR3557493.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3557493.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4891869
Sequences flagged as poor quality0
Sequence length125
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT619221.2658147632326213No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT311990.6377725977535376No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT238700.48795255964540346No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT120440.24620446704521318No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA103690.21196397532313316No Hit
GTTAATGATAGTGTGTCGAAACACACTGGGTTTCCCCATTCGGAAATCGC85790.17537264387088042No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT84840.1734306458329117No Hit
ATCATTAACTGAATCCATAGGTTAATGAGGCGAACCGGGGGAACTGAAAC84780.17330799332525051No Hit
GTACATGGGAAGCAGTGGTATCAAAAAAAAAAAAAAAAAAAAAAAAAAAA78640.16075655337458955No Hit
GTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGAGGATGGT62670.12811054425210489No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT61890.1265160616525095No Hit
GTGGATGCCCTGGCAGTCAGAGGCGATGAAGGACGTGCTAATCTGCGATA58750.12009724708490763No Hit
GTACATGGGCTTTTGTGATGAAGATGGGAGGGGGAAAAAAAAAAAAAAAA54840.11210439200232059No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT50720.1036822531429194No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCATAG13200.043.7381021
TAACGCA11450.041.57664
ATAGTAC15900.037.799734
AGATTAC21250.037.2440722
AACGCAG669850.035.319946
GTATCAA670400.034.0747831
GACCGTT9150.033.8106467
ATAACGC15950.033.577343
ACGGTAC11850.032.6406973
CATGGGT66700.032.2958834
GTATAAC14050.032.1958121
GGACCGT14850.032.0563966
TATCACG8750.031.9634952
CGCAGAG751650.031.5257848
ACGCAGA749600.031.5088217
ACATGGG631400.030.997293
TCACGCA9050.030.9039334
GATTACT25750.030.5043053
GTACATG665000.030.4491231
TACATGG667850.030.3212762