FastQCFastQC Report
Fri 10 Feb 2017
SRR3557489.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3557489.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4680193
Sequences flagged as poor quality0
Sequence length125
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT503211.0751907026056404No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT242540.5182264919416785No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT200970.4294053685392889No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT88640.18939389892681777No Hit
GTACATGGGAAGCAGTGGTATCAAAAAAAAAAAAAAAAAAAAAAAAAAAA56760.1212770498994379No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT49530.10582896901901269No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA450150.041.8868031
AACGCAG473700.040.7860956
ACGCAGA540000.035.6306537
TATCAAC527400.035.618612
ATCAACG528700.035.4409943
CGCAGAG543600.035.4275258
CGCATAG9750.035.4056551
TCAACGC544150.034.8721244
CAACGCA547600.034.6951455
GCAGAGT574900.033.5193949
TAACGCA8350.032.0677534
ACATGGG465750.031.7990763
GTACATG474300.031.7731171
TACATGG476600.031.5371042
CATGGGT53000.029.1903934
CATGGGG146350.028.7047984
AGAGTAC564550.027.13640810-11
CATGGGC126850.026.3156994
GGACCGT12450.026.2861256
GTACTGG34200.026.2784711
Produced by FastQC (version 0.11.5)