FastQCFastQC Report
Fri 10 Feb 2017
SRR3557477.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3557477.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6166920
Sequences flagged as poor quality0
Sequence length125
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT476750.7730763492959208No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT245310.39778365861726755No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT185290.3004579271338042No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT91270.14799932543311736No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA464650.038.9777031
AACGCAG485600.038.5711366
ACGCAGA552550.033.7549257
ATCAACG541800.033.3515243
CGCAGAG560800.033.3220028
GTACATG527250.033.232381
TCAACGC552300.033.202254
ACATGGG517800.033.0013963
CAACGCA557550.032.996065
TATCAAC548300.032.977852
TACATGG533550.032.7743722
CGCATAG13300.032.6671681
TAACGCA10450.031.8846514
GCAGAGT601650.031.079339
CATGGGG181400.029.7495174
CATGGGT70350.028.0791454
GGACCGT14800.027.3371736
AGAGTAC582250.025.85660710-11
ACAACGC11300.025.2739283
CATGGGA263700.025.0902274
Produced by FastQC (version 0.11.5)