FastQCFastQC Report
Fri 10 Feb 2017
SRR3557476.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3557476.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3647175
Sequences flagged as poor quality0
Sequence length125
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT422581.158650188159329No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT223600.6130772447168014No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT173750.47639611480118177No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT81930.22463961833473853No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA79200.21715437290505665No Hit
ATCATTAACTGAATCCATAGGTTAATGAGGCGAACCGGGGGAACTGAAAC66770.18307320049079082No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT66580.1825522493436701No Hit
GTTAATGATAGTGTGTCGAAACACACTGGGTTTCCCCATTCGGAAATCGC64050.17561537354253634No Hit
GTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGAGGATGGT59120.1620980621988251No Hit
GTACATGGGAAGCAGTGGTATCAAAAAAAAAAAAAAAAAAAAAAAAAAAA53820.14756626704229986No Hit
GTGGATGCCCTGGCAGTCAGAGGCGATGAAGGACGTGCTAATCTGCGATA49910.13684564080418407No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT41550.11392379033087253No Hit
ATACAGGGTGACAGCCCCGTACACAAAAATGCACATGCTGTGAGCTCGAT40890.11211417055666373No Hit
GGATACCACGTGTCCCGCCCTACTCATCGAGCTCACAGCATGTGCATTTT39470.10822074619397204No Hit
GTACATGGGCTTTTGTGATGAAGATGGGAGGGGGAAAAAAAAAAAAAAAA39240.1075901211211417No Hit
CCTATATAGTATAGCTTCCCATCTTCTTTGAGAGTTGTTGGTTTATGCTC37640.10320316409275672No Hit
ATATAGTATAGCTTCCCATCTTCTTTGAGAGTTGTTGGTTTATGCTCATC37180.10194191394709604No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAACGCA8100.044.077394
GACCGTT5500.040.0236937
TACAACG6500.038.4490432
CGCATAG11150.037.900091
GGACCGT12700.036.543556
AACGCAG495950.034.192186
TACTGGT27200.034.1275632
ACAACGC7500.034.11593
GTACTGG31800.033.690091
GTATCAA484150.033.4753041
CATGGGT51800.033.3133134
ATAGTAC13200.032.9077724
GGTTCAC28450.031.5801756
AGATTAC18100.031.231632
CGCAGAG551350.030.7104138
ACGCAGA550200.030.6448427
ACATGGG467200.030.4017933
GTATAAC12000.030.2555711
ATGGGTG31750.029.9844515
TACATGG497650.029.9166452
Produced by FastQC (version 0.11.5)