FastQCFastQC Report
Fri 10 Feb 2017
SRR3557474.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3557474.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5239418
Sequences flagged as poor quality0
Sequence length125
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT982591.87538005175384No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT480080.9162849766901592No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT361380.6897330963095519No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT179360.3423280982735105No Hit
GTACATGGGAAGCAGTGGTATCAAAAAAAAAAAAAAAAAAAAAAAAAAAA127600.24353849988681947No Hit
GTTAATGATAGTGTGTCGAAACACACTGGGTTTCCCCATTCGGAAATCGC110780.21143569762901146No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA101090.19294127706550615No Hit
ATCATTAACTGAATCCATAGGTTAATGAGGCGAACCGGGGGAACTGAAAC100940.1926549857255138No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT98180.1873872250696547No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT91850.17530573052197782No Hit
GTATCAACGCAGAGTACATGGGCTTTTGTGATGAAGATGGGAGGGGGAAA91420.17448502868066645No Hit
GTACATGGGCTTTTGTGATGAAGATGGGAGGGGGAAAAAAAAAAAAAAAA79960.15261237030525146No Hit
GTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGAGGATGGT75260.14364190831882473No Hit
GTGGATGCCCTGGCAGTCAGAGGCGATGAAGGACGTGCTAATCTGCGATA71710.13686634660567262No Hit
GCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT68750.13121686416315706No Hit
GTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAAAAAAAAAAAAAAA67600.12902196388988244No Hit
GTACATGGGGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAA63200.12062408458344037No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT58730.11209260265166857No Hit
ATACAGGGTGACAGCCCCGTACACAAAAATGCACATGCTGTGAGCTCGAT53920.10291219368258077No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCATAG13350.046.3647351
GACCGTT9950.044.2473077
AACGCAG907550.041.0947536
GTATCAA897200.040.5841031
TAACGCA11750.036.9675984
CGCAGAG1026000.036.3698128
ATAGTAC17550.036.277964
ACGCAGA1028550.036.244597
GGACCGT18900.035.8897936
TACAACG7850.035.6257862
TTAGGAC14650.035.3360373
TATCAAC1033150.035.1377452
GCAGAGT1068250.034.9202239
GTATAAC12900.034.602481
AGATTAC23050.034.5915572
ATCAACG1048450.034.52853
TCAACGC1079900.033.9196434
CAACGCA1091700.033.6123245
CATGGGT64950.033.2555164
GCATAGT18800.033.2328572
Produced by FastQC (version 0.11.5)