FastQCFastQC Report
Fri 10 Feb 2017
SRR3557471.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3557471.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8527853
Sequences flagged as poor quality0
Sequence length125
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT892321.0463594998647372No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT451620.5295822993196528No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT329830.3867679238842414No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT170720.20019106802145864No Hit
GTACATGGGAAGCAGTGGTATCAAAAAAAAAAAAAAAAAAAAAAAAAAAA103580.12146081786353494No Hit
GTTAATGATAGTGTGTCGAAACACACTGGGTTTCCCCATTCGGAAATCGC97110.11387391410241242No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT88080.10328508242344234No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA795700.041.9594761
AACGCAG827450.040.973026
ACGCAGA935900.036.1393287
CGCAGAG941950.035.9640588
TATCAAC938000.035.4720842
ATCAACG944550.035.037123
TCAACGC965750.034.6561364
CAACGCA973550.034.463645
GCAGAGT999550.033.9273079
GTACATG839100.033.434321
ACATGGG827450.033.1282733
TACATGG846200.033.083312
CATGGGG270300.030.9280574
TAACGCA14050.028.3740184
CATGGGC215200.028.2020234
CATGGGT92250.027.928264
AGAGTAC979950.027.90023810-11
CGCATAG15900.026.949581
CATGGGA412550.026.2059974
GTACTGG55750.026.047231
Produced by FastQC (version 0.11.5)