FastQCFastQC Report
Fri 10 Feb 2017
SRR3557470.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3557470.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4069710
Sequences flagged as poor quality0
Sequence length125
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT464281.1408183875509559No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT234260.5756184101569891No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT176730.43425698637003624No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT90220.2216865575188404No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA73180.1798162522636748No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT60540.14875752817768342No Hit
GTACATGGGAAGCAGTGGTATCAAAAAAAAAAAAAAAAAAAAAAAAAAAA56310.1383636671900455No Hit
GTTAATGATAGTGTGTCGAAACACACTGGGTTTCCCCATTCGGAAATCGC50660.12448061409781042No Hit
ATCATTAACTGAATCCATAGGTTAATGAGGCGAACCGGGGGAACTGAAAC48050.11806738072245933No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT47680.11715822503323332No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCATAG9850.040.483351
AACGCAG498000.035.1015976
TAACGCA6950.035.1001364
GTATCAA502800.033.9722631
ACGGTAC7950.033.6786843
CATGGGT51450.031.5709044
ACGCAGA559200.031.1934817
CGCAGAG560800.031.1893548
ACATGGG483950.030.773023
TATCACG5650.030.5393162
GTACATG509550.030.4620151
ATAGTAC13900.030.391584
TACATGG513800.030.1663912
GCAGAGT591600.029.615859
CATGGGG127500.029.4461774
AGATTAC16400.029.386712
CATGGGC114450.029.3725994
TATCAAC577150.029.3706442
ATCAACG579050.029.2228973
TCAACGC590750.029.0772134