FastQCFastQC Report
Fri 10 Feb 2017
SRR3557460.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3557460.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7588807
Sequences flagged as poor quality0
Sequence length125
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT1182091.5576756662806157No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT643190.8475508732795549No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT446480.5883401699371192No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT233760.30803260644262004No Hit
GTACATGGGAAGCAGTGGTATCAAAAAAAAAAAAAAAAAAAAAAAAAAAA177790.23427924837197733No Hit
GTTAATGATAGTGTGTCGAAACACACTGGGTTTCCCCATTCGGAAATCGC131470.17324198652041092No Hit
ATCATTAACTGAATCCATAGGTTAATGAGGCGAACCGGGGGAACTGAAAC130100.1714366961763555No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT120260.1584702312234321No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA102940.1356471445380018No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT96800.12755628124420612No Hit
GTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGAGGATGGT96500.1271609621907633No Hit
GTATCAACGCAGAGTACATGGGCTTTTGTGATGAAGATGGGAGGGGGAAA94580.12463092024872949No Hit
GCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT92970.12250937466191986No Hit
GTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAAAAAAAAAAAAAAA91040.11996615541810457No Hit
GTGGATGCCCTGGCAGTCAGAGGCGATGAAGGACGTGCTAATCTGCGATA88020.11598661028011385No Hit
GTACATGGGCTTTTGTGATGAAGATGGGAGGGGGAAAAAAAAAAAAAAAA85920.11321937690601433No Hit
GTACATGGGGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAA85440.11258686642050589No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT82640.10889722192170655No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT77270.10182101086508064No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCATAG17450.041.267781
AACGCAG1035950.040.9639786
GTATCAA1078550.038.4659081
ACGCAGA1172850.036.1166157
CGCAGAG1175900.036.063418
CATGGGT83650.034.5709534
GCAGAGT1236600.034.2931949
TATCAAC1228350.033.66212
CATGGGG253850.033.23844
ATCAACG1245300.033.0557943
ACATGGG953100.032.876343
GTACATG981300.032.6591641
TCAACGC1280000.032.5873534
TACATGG983050.032.5586972
CAACGCA1286650.032.496695
ATAGTAC23100.032.4563454
GACCGTT13450.032.290157
TAACGCA16500.031.0138424
GTATAAC18250.029.3495081
ATGGGTG57350.029.2580435
Produced by FastQC (version 0.11.5)