FastQCFastQC Report
Fri 10 Feb 2017
SRR3557454.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3557454.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2965538
Sequences flagged as poor quality0
Sequence length125
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT444961.500436008575847No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT239790.8085885259268302No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT175380.591393534663862No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT84200.28392824506042413No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA71040.23955181150941246No Hit
GTACATGGGAAGCAGTGGTATCAAAAAAAAAAAAAAAAAAAAAAAAAAAA63300.21345199420813357No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT54700.18445219720671258No Hit
ATCATTAACTGAATCCATAGGTTAATGAGGCGAACCGGGGGAACTGAAAC45520.15349659994240505No Hit
GTTAATGATAGTGTGTCGAAACACACTGGGTTTCCCCATTCGGAAATCGC43540.1468199024932407No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT41900.14128970864645807No Hit
GTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGAGGATGGT41280.13919902560682074No Hit
GTACATGGGCTTTTGTGATGAAGATGGGAGGGGGAAAAAAAAAAAAAAAA40110.13525370438686No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT34250.11549337759286847No Hit
GTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATG33400.11262711858691407No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCATAG8350.054.8874361
ATAGTAC12100.038.368784
AGATTAC15900.038.1831472
TACAACG6600.036.9750372
TAACGCA7900.036.917964
GATTACT16550.036.3238683
TAGTACT13300.036.2495045
TCACGCA5250.035.145614
AACGCAG489500.034.634736
GCATAGT13600.034.136932
GTATCAA488600.033.6464231
GACCGTT4100.033.370577
CATGGGG109400.033.296824
TATCACG6000.032.736442
CATGGGC98800.032.531614
TACTGGT20550.032.1499182
ATCTACG5000.032.141232
CATGGGT47650.031.9775924
ACATGGG454400.031.6466223
GTACTGG22750.031.3955821
Produced by FastQC (version 0.11.5)