FastQCFastQC Report
Fri 10 Feb 2017
SRR3557451.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3557451.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2549942
Sequences flagged as poor quality0
Sequence length125
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT291411.1428103070579645No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT143070.5610715851576232No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT114330.4483631392400298No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT52130.20443602246639336No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA35720.14008161754267354No Hit
GTACATGGGAAGCAGTGGTATCAAAAAAAAAAAAAAAAAAAAAAAAAAAA34490.13525797841676399No Hit
GTTAATGATAGTGTGTCGAAACACACTGGGTTTCCCCATTCGGAAATCGC32840.12878724300395852No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT27700.108629921778613No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT27660.10847305546557529No Hit
ATCATTAACTGAATCCATAGGTTAATGAGGCGAACCGGGGGAACTGAAAC26010.10200232005276982No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCATAG6200.041.2773321
GTATCAA276650.039.713291
AACGCAG291700.039.009146
CATGGGT38500.034.782174
ACGCAGA326050.034.753517
CGCAGAG327850.034.68978
ACATGGG291700.034.297793
GTACATG302500.034.0766871
TACATGG304650.033.83622
ATCAACG324850.033.6192553
TATCAAC327950.033.3922042
TCAACGC333200.033.36624
CATGGGG92400.033.0430644
GCAGAGT345350.033.0007559
CAACGCA338400.032.938835
GTATAAC7850.032.6012081
TAACGCA6400.031.6179584
CATGGGC66800.030.3817524
TACAACG3400.029.7580782
ATAGTAC8850.028.9174564