FastQCFastQC Report
Fri 10 Feb 2017
SRR3557450.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3557450.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7623378
Sequences flagged as poor quality0
Sequence length125
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT856851.123976798736728No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT433060.5680683812346705No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT323840.42479856042819863No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT149190.19570064609153578No Hit
GTACATGGGAAGCAGTGGTATCAAAAAAAAAAAAAAAAAAAAAAAAAAAA100250.13150338340824763No Hit
GTTAATGATAGTGTGTCGAAACACACTGGGTTTCCCCATTCGGAAATCGC87230.11442434049577498No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT81970.10752451209949186No Hit
ATCATTAACTGAATCCATAGGTTAATGAGGCGAACCGGGGGAACTGAAAC80600.10572740850578315No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA739400.042.822481
AACGCAG776350.041.5592886
ACGCAGA883050.036.4433177
CGCAGAG885600.036.3518188
TATCAAC874200.036.1169742
ATCAACG874750.035.97863
TCAACGC896200.035.515934
CAACGCA907450.035.132815
GCAGAGT938850.034.2900059
GTACATG762550.031.3525681
ACATGGG745100.031.2079753
TACATGG767950.031.0854022
CGCATAG14950.031.0522421
CATGGGG231650.029.7260914
AGAGTAC927350.028.22697310-11
CATGGGT94400.027.4254654
CATGGGC191150.026.5589854
AGTACTT536900.026.32997112-13
TAACGCA13100.025.4421314
TACAACG14300.024.5557232
Produced by FastQC (version 0.11.5)