FastQCFastQC Report
Fri 10 Feb 2017
SRR3557443.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3557443.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5097466
Sequences flagged as poor quality0
Sequence length125
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT494520.9701290798212288No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT249430.4893215570246079No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT185910.3647106228859594No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT84960.16667104792851978No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA81480.1598441264738205No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT68350.13408623029560177No Hit
ATCATTAACTGAATCCATAGGTTAATGAGGCGAACCGGGGGAACTGAAAC62940.12347311389619862No Hit
GTTAATGATAGTGTGTCGAAACACACTGGGTTTCCCCATTCGGAAATCGC56520.11087862086770171No Hit
GTACATGGGAAGCAGTGGTATCAAAAAAAAAAAAAAAAAAAAAAAAAAAA53720.105385695559323No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCATAG10350.036.803021
GTATCAA532800.035.6456381
AACGCAG545300.035.63956
TAACGCA8250.033.906874
ACGCAGA603350.032.131657
CGCAGAG608500.031.9965658
ATCAACG613150.030.7512133
TATCAAC615650.030.694012
TCAACGC626350.030.4357264
ACATGGG517700.030.4082433
GCAGAGT643000.030.2983029
CAACGCA629150.030.2967055
CATGGGG157500.030.1176834
CATGGGT59650.029.93334
GTACATG555350.029.6756321
TACATGG556950.029.5262642
CATGGGC115950.026.6403794
ACAACGC8800.026.377023
AGATTAC23000.025.8771232
ACGGTAC9950.025.721083
Produced by FastQC (version 0.11.5)