FastQCFastQC Report
Fri 10 Feb 2017
SRR3557442.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3557442.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2128895
Sequences flagged as poor quality0
Sequence length125
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT278641.3088480173986974No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT132500.6223886100535725No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT105170.4940121518440318No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA56200.2639867161132888No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT52550.24684167138351115No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT43360.20367373684470114No Hit
GTACATGGGAAGCAGTGGTATCAAAAAAAAAAAAAAAAAAAAAAAAAAAA35050.16463940213115255No Hit
GTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATG29720.13960293955314845No Hit
ATCATTAACTGAATCCATAGGTTAATGAGGCGAACCGGGGGAACTGAAAC29640.13922715775085198No Hit
GTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGAGGATGGT27040.12701424917621582No Hit
GTTAATGATAGTGTGTCGAAACACACTGGGTTTCCCCATTCGGAAATCGC26870.12621571284633576No Hit
GTACATGGGCTTTTGTGATGAAGATGGGAGGGGGAAAAAAAAAAAAAAAA26050.12236394937279668No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT24280.11404977699698671No Hit
GCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGT23380.10982223172115112No Hit
GTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGAAGCAGTGGTATC23100.10850699541311339No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT22940.10775543180852039No Hit
GAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGG22240.10446734103842603No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TACAACG4950.055.3214842
ACAACGC5500.051.9540823
TAACGCA6650.043.8647424
CGCATAG5850.042.7400051
GACCGTT3050.040.9575587
GTATAAC8250.038.965561
TATCACG3550.038.56922
GTACAAC7650.038.130791
ATAACGC9550.037.40153
AGATTAC10800.035.2774662
CATGGGT39800.033.9534534
GTACTGG15800.033.5331271
AACGCAG337400.032.704956
CATGGGG80500.032.6864364
ATGGGTG23300.031.934835
GTATCAA331250.031.8455661
CATGGGC66000.031.3889264
GGACCGT6350.030.9139756
TACTGGT15600.030.5285742
TCACGCA4300.030.4575774