FastQCFastQC Report
Fri 10 Feb 2017
SRR3557441.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3557441.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4820511
Sequences flagged as poor quality0
Sequence length125
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT571541.1856419371307316No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT289000.5995215030107804No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT220200.45679804485458075No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT103520.21474901727223528No Hit
GTACATGGGAAGCAGTGGTATCAAAAAAAAAAAAAAAAAAAAAAAAAAAA74060.15363516440476954No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT53510.1110048291560791No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA49030.10171120862497772No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AACGCAG530800.039.6143156
CGCATAG12050.038.5351681
GTATCAA532900.038.3734361
ACGCAGA602000.034.879637
CGCAGAG603950.034.796568
GCAGAGT635400.033.055539
ATCAACG622100.032.632013
TATCAAC626200.032.5324362
TCAACGC638750.032.303334
CAACGCA642500.032.185245
ACATGGG486000.031.8850843
GTACATG503050.031.7984661
TACATGG507700.031.49552
ATAGTAC15850.030.4232374
CATGGGT59500.029.9160254
CATGGGG146300.029.7862914
TAGTACT17100.028.1960965
CATGGGC125100.027.5531274
AGAGTAC628300.027.38419310-11
GGACCGT11350.027.2521726
Produced by FastQC (version 0.11.5)