FastQCFastQC Report
Fri 10 Feb 2017
SRR3557439.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3557439.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1468699
Sequences flagged as poor quality0
Sequence length125
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT144740.9854980496344043No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT73120.4978555851130831No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT60950.4149931333785888No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT27000.18383617065171284No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA23970.16320566705635395No Hit
GTACATGGGAAGCAGTGGTATCAAAAAAAAAAAAAAAAAAAAAAAAAAAA22160.15088183487562803No Hit
ATCATTAACTGAATCCATAGGTTAATGAGGCGAACCGGGGGAACTGAAAC18590.12657460786723487No Hit
GTTAATGATAGTGTGTCGAAACACACTGGGTTTCCCCATTCGGAAATCGC18050.1228978844542006No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT17350.11813176151137844No Hit
GTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGAGGATGGT16620.11316137615672102No Hit
GTACATGGGCTTTTGTGATGAAGATGGGAGGGGGAAAAAAAAAAAAAAAA15120.10294825556495918No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAACGCA4150.050.200684
CGCATAG3850.046.3820761
GTATAAC5050.045.9687651
TATCACG3250.045.787432
TACAACG3650.040.7696342
TCACGCA4150.040.160544
ATAACGC5500.040.043193
ATCACGC3850.038.651733
AAACGCA5350.037.828125
ACAACGC3950.037.6732033
AACGCAG174400.034.721236
AGATTAC7850.032.6053162
GTATCAA174350.031.8870641
CGCAGAG193950.031.344048
ACGCAGA193400.031.2793927
ACATGGG168750.031.2521273
GTACATG178400.030.9296151
TACATGG179500.030.5742682
CATGGGG48350.030.285054
GCAGAGT203650.029.8803049