FastQCFastQC Report
Fri 10 Feb 2017
SRR3557438.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3557438.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4211770
Sequences flagged as poor quality0
Sequence length125
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT334600.7944403421839275No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT163070.38717688762681723No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT131180.31146050235411715No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT57100.1355724552860199No Hit
GTTAATGATAGTGTGTCGAAACACACTGGGTTTCCCCATTCGGAAATCGC48110.11422751004921922No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA326800.040.9672851
AACGCAG347550.039.451986
ACGCAGA392450.034.953467
CGCAGAG397050.034.5934528
ATCAACG383400.034.5463833
TCAACGC392550.034.241564
TATCAAC389350.034.1866262
CAACGCA394800.034.179285
ACATGGG355650.032.9067763
GCAGAGT421250.032.634379
GTACATG370050.032.5435751
TACATGG371600.032.327352
CATGGGG124650.031.3760094
GGACCGT9100.030.723576
TAACGCA7400.030.5686684
CATGGGT52050.030.1931084
TATCACG5000.028.5736622
AGAGTAC413250.026.79571510-11
GACCGTT6800.026.2438477
CGCATAG8750.025.1723331
Produced by FastQC (version 0.11.5)