FastQCFastQC Report
Fri 10 Feb 2017
SRR3557433.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3557433.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1370401
Sequences flagged as poor quality0
Sequence length125
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT168581.2301508828437808No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT81660.5958839784851295No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT69650.5082453967853205No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT32650.238251431515301No Hit
GTACATGGGAAGCAGTGGTATCAAAAAAAAAAAAAAAAAAAAAAAAAAAA25870.18877686166311905No Hit
GTACATGGGCTTTTGTGATGAAGATGGGAGGGGGAAAAAAAAAAAAAAAA20290.1480588528467215No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA19750.14411840038061852No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT17170.12529179415368202No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT14830.10821650013390241No Hit
GTACATGGGGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAA14370.10485981840351838No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCATAG4200.065.187481
TAACGCA3650.048.9197274
ATAGTAC5900.046.4046444
ATAACGC5050.044.7865753
GCATAGT6400.042.779282
GTATAAC4200.041.0964551
AACGCAG173350.038.090536
TAGTACT7400.036.996955
ACATGGG178450.035.2211043
TACATGG181500.035.153922
GTACATG181850.035.02081
GTATCAA186300.034.1203961
CGCAGAG196700.033.568858
CATGGGC51100.033.4284824
ACGCAGA198100.033.331627
CATGGGT18450.032.904814
CATGGGG44550.032.865714
AGATTAC7250.032.0171172
GCAGAGT207200.031.8964399
CATAGTA9150.030.5726013