FastQCFastQC Report
Fri 10 Feb 2017
SRR3557432.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3557432.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4463981
Sequences flagged as poor quality0
Sequence length125
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT615111.3779404527035397No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT294540.6598146363078158No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT233830.5238149535134671No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT107530.24088364175385157No Hit
GTTAATGATAGTGTGTCGAAACACACTGGGTTTCCCCATTCGGAAATCGC75690.16955717329442038No Hit
GTACATGGGAAGCAGTGGTATCAAAAAAAAAAAAAAAAAAAAAAAAAAAA70590.1581323934846497No Hit
ATCATTAACTGAATCCATAGGTTAATGAGGCGAACCGGGGGAACTGAAAC68130.15262161734111324No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA63760.14283214915117246No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT57790.12945843631502912No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT54220.12146109044818963No Hit
GTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGAGGATGGT53930.12081144610606542No Hit
GTGGATGCCCTGGCAGTCAGAGGCGATGAAGGACGTGCTAATCTGCGATA50310.1127020925940321No Hit
GTATCAACGCAGAGTACATGGGCTTTTGTGATGAAGATGGGAGGGGGAAA48410.10844580207666654No Hit
GTACATGGGCTTTTGTGATGAAGATGGGAGGGGGAAAAAAAAAAAAAAAA45110.10105329749387373No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA573850.040.5008051
AACGCAG591450.040.0283436
GACCGTT6600.039.656227
ACGCAGA670450.035.187547
CGCAGAG673600.035.049488
TATCAAC662550.034.9962772
CATGGGT72900.034.9411934
ATCAACG666200.034.7241363
TCAACGC682650.034.1837964
CAACGCA685900.034.0357445
GCAGAGT706500.033.4088949
ACATGGG562950.032.8362163
GTACATG587400.032.4536821
TACATGG588950.032.265772
GGACCGT12850.031.9410026
TAACGCA8700.031.4673464
ACGGTAC11450.031.1865483
ATGGGTG43150.030.8919265
TCACGCA7800.030.5201684
CATGGGC125650.030.3137054
Produced by FastQC (version 0.11.5)