FastQCFastQC Report
Fri 10 Feb 2017
SRR3557431.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3557431.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3568550
Sequences flagged as poor quality0
Sequence length125
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT379451.0633170335290245No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT187780.5262081237477406No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT145690.4082610584130809No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT70920.19873618136217794No Hit
GTACATGGGAAGCAGTGGTATCAAAAAAAAAAAAAAAAAAAAAAAAAAAA50840.14246682826358045No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA50090.14036513429824438No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT43690.12243067912737667No Hit
GTTAATGATAGTGTGTCGAAACACACTGGGTTTCCCCATTCGGAAATCGC42920.12027293998963164No Hit
ATCATTAACTGAATCCATAGGTTAATGAGGCGAACCGGGGGAACTGAAAC39440.11052107999047231No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT36800.10312311723248938No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TACAACG6000.040.673772
AACGCAG402500.037.242826
GTATCAA397650.036.643621
CGCATAG9600.034.1019671
ACGCAGA452300.033.129097
CGCAGAG454300.033.1272748
TATCAAC459700.031.5287172
ATCAACG459650.031.506253
GCAGAGT481250.031.2968679
TCAACGC472800.030.9950564
CAACGCA477850.030.8019245
GTATAAC10250.030.7779671
ACATGGG371150.030.583213
TAACGCA8400.030.46994
CATGGGG104850.030.2580324
GTACATG390500.029.9674911
ACAACGC7550.029.9583933
TACATGG392500.029.829542
ATAGTAC12400.028.8012544
GGTTTCA20050.027.609371