FastQCFastQC Report
Fri 10 Feb 2017
SRR3557429.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3557429.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4616889
Sequences flagged as poor quality0
Sequence length125
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT485581.0517471830056992No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT240310.5205020090368212No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT177120.38363495418668286No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT87970.19053956029698788No Hit
GTACATGGGAAGCAGTGGTATCAAAAAAAAAAAAAAAAAAAAAAAAAAAA58280.1262321879516705No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA57750.1250842287956241No Hit
GTTAATGATAGTGTGTCGAAACACACTGGGTTTCCCCATTCGGAAATCGC54400.11782826054514198No Hit
ATCATTAACTGAATCCATAGGTTAATGAGGCGAACCGGGGGAACTGAAAC51530.11161195341711702No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT47090.10199508803438852No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AACGCAG473850.039.2171136
GTATCAA462850.038.98451
TAACGCA8850.036.984734
ACGCAGA533500.034.7765437
CGCAGAG537200.034.581318
TATCAAC537050.033.454262
ATCAACG536750.033.384263
CGCATAG10900.033.3047681
TCAACGC549850.033.1408774
CAACGCA554400.032.9529765
GCAGAGT570650.032.6063659
ACATGGG452550.032.1656953
GTACATG474450.031.596631
TACATGG476550.031.3824632
CATGGGG144300.031.2199764
CATGGGT53100.028.803264
AGAGTAC557400.026.850310-11
CATGGGC109700.026.7992954
ACAACGC9100.025.5050543
AGTACTT321750.025.46690212-13
Produced by FastQC (version 0.11.5)